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FLAG-tagged BRD4 proteins were incubated with 500 ng of refolded RNA while rotating at 4 °C for 1 h in RNA binding buffer (20 mM Tris-HCl at pH 7.4, 100 mM KCl, 0.2 mM EDTA, 0.05% NP40, 0.4 U RNase inhibitor, PICs)

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A Combined NMR and MALDI Approach F.Samperi, M.S.Montaudo, C.Puglisi, R.Alicata, G.Montaudo Macromolecules 36(19), 7143 - 2003 A combination of NMR and MALDI was found to be a suitable tool to study the exchange reactions that occur during the melt mixing of Nylon-6 and poly(butylene terephthalate) (Ny6/PBT) blends